In the presence of glucose and fructose, amylase production was pretty much nil and that was a proof that glucose and fructose repressed amylase synthesis by L. This observation is in agreement with the reports of Theodoro and Martin displaying that synthesis of carbohydrate degrading enzymes in some microbial species leads to catabolic repression by substrate such as glucose and fructose. Related results have been observed by Halsetine et al. for the production of amylase by the hyperthemophilic archeon Sulfolobus solfataricus. From the use of diverse carbohydrate sources in the present study, soluble starch proved to be the finest inducer of amylase production . In the presence of soluble starch at concentration of 1% (w/v), the enzyme yield reached 107.0±1.2 U/ml after 48 hours of fermentation, whilst in the presence of raw cassava starch at the same concentration, the enzyme yield was 67.1±0.5 U/ml. These results are in agreement with the reports of Cherry et al. , Saxena et al. who reported maximum amylase production when starch was employed as carbohydrate supply. The outstanding properties of the pure enzyme have been characterized and the kinetic parameters according to Lineweaver-Burk plot were calculated. α-Amylase activity from the halophilic archaeon Haloferax mediterranei. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Horikoshi K. Production of alkaline amylase by alkalophilic microorganisms II Alkaline amylase produced by Bacillus no A-40–2. stearothermophilus US100. Saccharomyces cerevisiae as nicely as other fungi and bacteria have been utilised to create α-amylase in particular in the meals sector simply because of its “Generally Recognised as Safe” status honoured by the U.S. Meals and Drug Administration . There are two kinds of naturally occuring alkaline environments in the globe. One, high Ca2+ environments (ground waters bearing high Ca2) and two, low Ca2+ environments . Soda lakes represent a particular variety of salt lake, which contain an alkaline sodium carbonate/bicarbonate fraction among the dominant salts. According to them, glucose prevented α-amylase gene expression and not only secretion of performed enzyme. enzymes.bio to the fact amylase yield is greater with amylose (92.three U/ml) as carbohydrate supply than with amylopectin (50.1 U/ml), the L. fermentum 04BBA19 amylase is a lot more effective for hydrolysis of alpha-1,4 linkages than these of alpha-1,6. The amylase production improved with the soluble starch concentration (Fig. 4), reaching a maximum (180.5 ± .three U/ml) at the concentration variety of 8-16 % (w/v). These optimum starch concentrations for amylase production by L.
Optimization of cellulase production by Aspergillus niger NCIM 1207. Structural and functional elements of chloride binding to Alteromonas haloplanctis alpha-amylase. Enzymes involved in the processing of starch to sugars. Investigation of the mechanisms of irreversible thermoinactivation of Bacillus stearothermophilus alpha-amylase. Structure of chicken muscle triose phosphate isomerase determined crystallographically at two.five angstrom resolution utilizing amino acid sequence information. Copyright © 2013 Yasser R. Abdel-Fattah et al. This is an open access short article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original perform is effectively cited. They are largely confined to dry regions with higher evaporation rates that facilitate salt accumulation in local depressions. .Molecular Cloning and Expression of a Thermostable α-Amylase from Geobacillus sp,Master’s Thesis, Universiti Putra Malaysia.
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